Insertion junction: CLIP2.024829_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.024829
Chromosome:chromosome 2
Location:4643230
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g107500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAGTAATGCCCACAGGCGCTACCCTGCGCACGCACCGGGTTGATCCTG
Internal bar code:CATGAGTCCCTGCTGCTGATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1745
LEAP-Seq percent confirming:92.1053
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCAATATAGGGCCAACA
Suggested primer 2:TGTGGATGACGGTGATGCTC