Insertion junction: CLIP2.024831_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.024831
Chromosome:chromosome 8
Location:1636217
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g365825 (1 of 1) 1.14.99.42 - Zeaxanthin 7,8-dioxygenase / Zeaxanthin 7,8(7',8')-cleavage dioxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGGAACGTAGTCTGCGCCTTCTGAACAATTCCTCAACTGCGCCTGTG
Internal bar code:ATGCGATGTGTGATGTGTGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:75
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTAAGGTGAGGTCCCCC
Suggested primer 2:ATGGTGCTGCTACTGCCAAT