| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024865 |
| Chromosome: | chromosome 16 |
| Location: | 837997 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801885 | (1 of 1) K15264 - putative methyltransferase [EC:2.1.1.-] (NSUN5, WBSCR20) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCACTGTTGCGCTGTCACTGCGGGAAAGTATGGGGATGGCTATGGGTT |
| Internal bar code: | GCATGAGCGATGGTGCATGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1625 |
| LEAP-Seq percent confirming: | 62.3188 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGTGCAGCTTTTGTTTA |
| Suggested primer 2: | GACCTGCCCAACACAAACAC |