| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.024887 |
| Chromosome: | chromosome 16 |
| Location: | 7059358 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674739 | (1 of 1) PF12537 - The Golgi pH Regulator (GPHR) Family N-terminal (GPHR_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAAATTGTGGACGTCATGGATGCCGGGTGTGTGCGTAGCGCGGGTCC |
| Internal bar code: | TAGTTGGATATGGGGAAGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTACGGCAACACACCCA |
| Suggested primer 2: | CTAGCACGGCGCAATCTCTA |