| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024894 |
| Chromosome: | chromosome 16 |
| Location: | 4609090 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670850 | (1 of 1) PTHR22975:SF2 - ECHINUS SPLICE FORM 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGCAAGAGCAACACTCCACTCATCTGGCACGCTGGCCGCCCTACCC |
| Internal bar code: | TGGGATACCGGCAGTGATGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1125 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAGTTTGGTGGTCTCCC |
| Suggested primer 2: | TGGGTGTGTGATCAAGGAGC |