Insertion junction: CLIP2.024909_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.024909
Chromosome:chromosome 8
Location:3831669
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g381516 (1 of 1) K11168 - dehydrogenase/reductase SDR family member 12 (DHRS12) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACAACCCTAGTCTCCTTCCTCTCACCTCCCACTCCTCTCACCTC
Internal bar code:TTGGCCACTTGAGCGGGCGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:252
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGCCTGAGACCGTGAAAC
Suggested primer 2:AACTTTCTCGTTCGGTCGCT