Insertion junction: CLIP2.024926_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.024926
Chromosome:chromosome 6
Location:6454284
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g293800 ARS16 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATAATATTTAACTCACCCTCCCCTCCCTCTGGCTCCAGGGCACCAACC
Internal bar code:AGTAGGTAGGTGGCCACACTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1924
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACGACTATCCCAGCGACTC
Suggested primer 2:GGGGGACAGGAAGGATTTGG