Insertion junction: CLIP2.024932_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.024932
Chromosome:chromosome 9
Location:1131525
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401551 BHD1 3-beta hydroxysteroid dehydrogenase/isomerase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGCATCCTACAGCGCCTTCGCTGCTGCCACCGCTACTGCTACTGCCC
Internal bar code:TAAACTGGTGACATAAGACGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2516
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGTCTGCTGTGTGTTTTG
Suggested primer 2:TATAGCGCCACACGTGTTGT