Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.024932 |
Chromosome: | chromosome 9 |
Location: | 1131525 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g401551 | BHD1 | 3-beta hydroxysteroid dehydrogenase/isomerase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCATCCTACAGCGCCTTCGCTGCTGCCACCGCTACTGCTACTGCCC |
Internal bar code: | TAAACTGGTGACATAAGACGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2516 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 48 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGGTCTGCTGTGTGTTTTG |
Suggested primer 2: | TATAGCGCCACACGTGTTGT |