| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.024938 |
| Chromosome: | chromosome 16 |
| Location: | 6355378 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675650 | ALDH6,ALD8,ALDH6B1 | (1 of 1) 1.2.1.27 - Methylmalonate-semialdehyde dehydrogenase (CoA acylating) / MSDH; Aldehyde dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCAACTAGCCCTCATCCTACATGCTGGCCTTGACCACACAGCCCCA |
| Internal bar code: | TTTGTGACGACACGAGTCATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2223 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCAGCGATTATGGTTTG |
| Suggested primer 2: | CTCCAAACCGCTCCAAGTCT |