Insertion junction: CLIP2.024957_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.024957
Chromosome:chromosome 13
Location:2127782
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g577450 GPA3 (1 of 1) 1.1.5.3 - Glycerol-3-phosphate dehydrogenase / sn-glycerol-3-phosphate dehydrogenase; Glycerol-3-phosphate acyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCCTGGGAAGGGGCGGGGAAGGGGGATAGCCGTTCATGTGAAGGGCG
Internal bar code:GTCATGCGTTTTGATAGAACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:69
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGCACACACACACATGC
Suggested primer 2:CGTGCGTGTCAGTGCATATG