Insertion junction: CLIP2.024981_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.024981
Chromosome:chromosome 4
Location:679823
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATCTAGACTTGAGCGTGTAGAAAACAACATAACACGCCACATTGTAT
Internal bar code:TGAGCAACAAGCTATGCACGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:5416
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:AATGCTGACGAGGCTCAACA
Suggested primer 2:CCTAATCCCCACACTTGCGT