Insertion junction: CLIP2.024983_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.024983
Chromosome:chromosome 12
Location:5400292
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g529250 (1 of 8) PTHR11339//PTHR11339:SF290 - EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED // PROTEIN T26A8.1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGAACGCTGCGCCAGCGCGCCGTGACGCACCATTGGATGCCTCGCCTA
Internal bar code:ACATACAAGATAATGGCCAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3215
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCAACTGCTCTTATGTCG
Suggested primer 2:CCAATCCTGGTGTCCTGCAT