| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.025011 |
| Chromosome: | chromosome 9 |
| Location: | 3627865 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396957 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGGCGCTCGCTGCCACCCCCGACCACAGCGCCCCAGTCACCTCAGCA |
| Internal bar code: | AACGTAATAAAGCTTGATGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1298 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGTATGTGGCATGGCA |
| Suggested primer 2: | GCAACCCGCCAGAAATTCTG |