Insertion junction: CLIP2.025014_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.025014
Chromosome:chromosome 16
Location:5066724
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g685800 (1 of 4) 3.1.2.2//3.1.2.20 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase // Acyl-CoA hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGCACACACAAAGCATACATCGCACACACTGTCTTTGCGCGACGTTTT
Internal bar code:ATAATTTCGCAAGCGAAGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1494
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCGTGCTGCTACTGCTAA
Suggested primer 2:CTTTGGGTCGTGACTCGTGA