| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025015 |
| Chromosome: | chromosome 12 |
| Location: | 8513016 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549400 | (1 of 4) PF09366 - Protein of unknown function (DUF1997) (DUF1997) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTGGTCGGTGCCCCGTGGCGGCGGTCCCCTTGCTGCGGTCTAAGAAG |
| Internal bar code: | GGTCTGAATTGTTGGAGGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTAAGGAGAGACAGGGT |
| Suggested primer 2: | CAAGAACTCTGTCGGCGTCT |