Insertion junction: CLIP2.025024_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.025024
Chromosome:chromosome 9
Location:3169235
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394436 (1 of 1) PF03030 - Inorganic H+ pyrophosphatase (H_PPase) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGCCTGCCTTGCCGGCGCTCGTACTCCCGCGCCTCAGCTTTCCGCTC
Internal bar code:TGAATGATCTGGTCATGCATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1129
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CACGTTGTCTGCGCACATAG
Suggested primer 2:CTTCCTACGACACAACCGCT