| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025059 |
| Chromosome: | chromosome 12 |
| Location: | 2854657 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503150 | (1 of 2) K17680 - twinkle protein [EC:3.6.4.12] (PEO1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGGGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTAGGGAGGC |
| Internal bar code: | GCGCCTGTGTGATGGGGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2086 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGCGCTGCAGATCATC |
| Suggested primer 2: | CGCTGATGTACTCGGTCTCC |