| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.025074 |
| Chromosome: | chromosome 7 |
| Location: | 5066922 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347800 | SMM29 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR14614:SF30 - S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTAAGAAAACAAGAGGCCGGGGGCCCGAGCGTGTGGGTGCGGAGCGA |
| Internal bar code: | AGAATTTTCGGACCAGATACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1461 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGTCGCAAACAGTTTCA |
| Suggested primer 2: | AGCAACAGTCCCAGATGTCG |