Insertion junction: CLIP2.025082_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025082
Chromosome:chromosome 14
Location:2646194
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g625450 VTE3 MPBQ/MSBQ methyltransferase, chloroplastic; (1 of 1) 2.1.1.295 - 2-methyl-6-phytyl-1,4-hydroquinone methyltransferase / MPBQ/MSBQ methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCATTGCCCGTACCATGCCTCGGCTTGTTTTTATACCCACACCCTTG
Internal bar code:TCTTTGGCTGGCGCTGTGCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2439
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGTGTTTGAGCCTG
Suggested primer 2:TGTCCAGTAAGACGCAACCC