Insertion junction: CLIP2.025087_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025087
Chromosome:chromosome 13
Location:850038
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g567363 FAP220 Flagellar Associated Protein 220; (1 of 14) PF02493 - MORN repeat (MORN) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGATGTCCCGGTACTTGGTCGAGGGGTGATCAATCTGGGGCCGTTCCG
Internal bar code:ATCTTGTTCTGCCAACTTTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2059
LEAP-Seq percent confirming:97.7778
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCGAAGCACTTCCCAGGT
Suggested primer 2:CGCCAAGCTCGGTAAATTGG