| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025101 |
| Chromosome: | chromosome 10 |
| Location: | 328435 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420200 | PHC55 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACAACAGGCACCACCACCGCGGGCACCACCACAGCGGGGTTGACGCC |
| Internal bar code: | TGTAGATATGCTCACGTTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1922 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTCCTTCTACGTCTCGC |
| Suggested primer 2: | CACAAGCTCTCTCACAGCCA |