| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.025111 |
| Chromosome: | chromosome 6 |
| Location: | 5564324 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287150 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGGATCCGGAGGGCCTAGCAGCAGCAGTCACAGCAGCAGAGGAAGT |
| Internal bar code: | CGTGATTAGACAATCAATTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2312 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTCAAACTCCAAGCTCC |
| Suggested primer 2: | ACTTGCGGCATTGTTTCCAC |