| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.025115 |
| Chromosome: | chromosome 10 |
| Location: | 5531217 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458216 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCCTGTCACGCTAGCCGGCAGCTTGGGGGACGCTGGCGGCAACGGGC |
| Internal bar code: | GAGGACGTAGATCATGCAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4717 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGCTATCTCACGGTCGC |
| Suggested primer 2: | GCGTGACAAGCCAAGTGTTT |