Insertion junction: CLIP2.025122_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.025122
Chromosome:chromosome 11
Location:3129669
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g475550 UMM10 (1 of 2) 2.1.1.165 - Methyl halide transferase / Methyl chloride transferase; methyl-halide synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACCGCTGCCCTTGAGAATGTGCCAAACAACTAAGGCGATGATACCGT
Internal bar code:TATAGAGAGCTAGGTTACGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4615
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGTTTGCAATGCGTGGT
Suggested primer 2:TCGCTGACGTTTCTGAGGAC