| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.025122 |
| Chromosome: | chromosome 11 |
| Location: | 3129778 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475550 | UMM10 | (1 of 2) 2.1.1.165 - Methyl halide transferase / Methyl chloride transferase; methyl-halide synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCCTGCCCCCATTGTCTACAACTGAGCATTCCCCGCGCCACGCACCT |
| Internal bar code: | TATAGAGAGCTAGGTTACGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1386 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTGACGTTTCTGAGGAC |
| Suggested primer 2: | GACTGTTTGCAATGCGTGGT |