Insertion junction: CLIP2.025131_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.025131
Chromosome:chromosome 7
Location:1304694
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g322350 (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGATCAACATATGATACTTAATACGATGTCTGCAGCGCGTTCAGAGAGGC
Internal bar code:ATACACAATAGGTTGTGTGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2059
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCTGCAACTCTGATTCCC
Suggested primer 2:ATCTATCTCAAGTGGCGCCG