Insertion junction: CLIP2.025135_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.025135
Chromosome:chromosome 12
Location:5909964
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533650 (1 of 1) PTHR13439//PTHR13439:SF0 - CT120 PROTEIN // BCDNA.GH12326 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAAAAAAAAGCGACCACCCACCTCACCTACCAGGAATTGCTTTCTGCC
Internal bar code:CAAAAGACGGCCTCGGCGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3437
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCACAAAGGTTGTCGAC
Suggested primer 2:CTGAGAGCGTTTGGGGACAT