| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.025137 |
| Chromosome: | chromosome 2 |
| Location: | 1598943 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085000 | (1 of 1) PTHR22847//PTHR22847:SF423 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTCTGCATCCGCATCGGACGCCGAGGCGCCCGCCACCTCCGCCGGCC |
| Internal bar code: | TTACAGGTTTACGAGCCGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2000 |
| LEAP-Seq percent confirming: | 59.2593 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCGTAGTCTGGTTGAGG |
| Suggested primer 2: | CAGTCTGAGGACGAGGAGGA |