| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.025140 |
| Chromosome: | chromosome 16 |
| Location: | 287433 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801864 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGCCGCTAACTGCGGTCAATAGCGTTGCCATGGGCGCTTCCTTTAGT |
| Internal bar code: | ATCCCCGTAGGTTTGTATTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 78.7879 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTCGAGCGAAATAGGGG |
| Suggested primer 2: | GTTGAGTTAGCCCTGCCTGT |