| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.025148 |
| Chromosome: | chromosome 17 |
| Location: | 5208993 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737084 | (1 of 1) PTHR15863//PTHR15863:SF2 - FAMILY NOT NAMED // UPF0544 PROTEIN C5ORF45 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCAACTCTGGCAAGGACATACGCCTCCACGTTCAGCGGCTCAACATG |
| Internal bar code: | GCGGTCAGCAAGAGGGTTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2753 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACTCCACCCTTACCGTG |
| Suggested primer 2: | GCAGCCCCCATGGATATCTC |