Insertion junction: CLIP2.025170_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025170
Chromosome:chromosome 9
Location:5238705
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g407050 MMP24 Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCAACCCCCACCGTCTACACTTAACGTACCGCCTCGCTCGCACCACCG
Internal bar code:CGGAATGTGTGTTACTCCCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:86
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGCTGGCATCTACAACCC
Suggested primer 2:CGCCTTGGGAACACACTTTG