| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.025173 |
| Chromosome: | chromosome 10 |
| Location: | 1305327 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427200 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATCATCCGGCAACCCAAACCCAAAGGTGCTGCGGCGGCAGCTGCTGC |
| Internal bar code: | AACGGATGTAGACTTTCTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1375 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACAAAGCCGGCCTTTCA |
| Suggested primer 2: | GACGTGCACATCATGGCATC |