Insertion junction: CLIP2.025187_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.025187
Chromosome:chromosome 10
Location:2374379
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g434850 (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAGGGGCAGGCATCTGCCTGGCCCGCGGTCCCCACACCTCCTAACAGA
Internal bar code:AGGGGTCGGGTAGGGGGGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2350
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCAACACCAACAGTAGC
Suggested primer 2:TAGGCACGTACAGAAGGGGA