Insertion junction: CLIP2.025216_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.025216
Chromosome:chromosome 6
Location:1136993
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g257800 POL1B,POL1-1 (1 of 2) IPR001098//IPR002298 - DNA-directed DNA polymerase, family A, palm domain // DNA polymerase A; DNA polymerase I, probably mitochondrial CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTCCGAGCTGCGCTCCGCGGTCCGCAGCGCCCGTGTGCTGCTGGTGG
Internal bar code:TGAGCTATATTCATCCGGCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1032
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGCAGAAATGGCGCAAA
Suggested primer 2:GAGATCAAACCTCACCGGGG