| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.025222 |
| Chromosome: | chromosome 16 |
| Location: | 6555497 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674250 | SRS10,SRS13 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 2) K12893 - splicing factor, arginine/serine-rich 4/5/6 (SFRS4_5_6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACCCTCAGGAATTTTGAAGTGAGTATCAAGCACTGGGCAACTGTCGA |
| Internal bar code: | TGGCTTTTCATAAAACCCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2479 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCTACTTAGGGGGTCGG |
| Suggested primer 2: | CATGTGTCATCCCCTGCACT |