Insertion junction: CLIP2.025226_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.025226
Chromosome:chromosome 1
Location:2816221
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g017400 (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCCTTGATCACAGGTACACTTAAAATACTGCGCGCGCGCGAGCCCCC
Internal bar code:TGCCTTTATGATTTCACTACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:502
LEAP-Seq percent confirming:28.9474
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CCGACATCGTGATCCTGGAG
Suggested primer 2:GGACGACACCTGCACACATA