Insertion junction: CLIP2.025226_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025226
Chromosome:chromosome 1
Location:2816228
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g017400 (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCAAAATCCACCGCCCACCGGCCCGCCGGCTCGACAGGCGCCGACGG
Internal bar code:CCCCAGGGGGGCAGGATGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2035
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGACACCTGCACACATA
Suggested primer 2:CCGACATCGTGATCCTGGAG