| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.025238 |
| Chromosome: | chromosome 17 |
| Location: | 2485929 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCCGCTCCCTGGCTGCAGGTGTGGTGCCAGCTAGCGTGCAGCTCACT |
| Internal bar code: | TCGTGATCATCTGTAAGAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1197 |
| LEAP-Seq percent confirming: | 36.3636 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATGACGTGATCTCGGAT |
| Suggested primer 2: | TCGCGTTATGGCTCCATACC |