| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025248 |
| Chromosome: | chromosome 6 |
| Location: | 2377655 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268250 | HTB9 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGCCTTGGCAGGTGCCTCGGCGCCGGCCTCGGCGGTCGCGGGCTTCT |
| Internal bar code: | GCCTTACTGCTGTCGGACGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3068 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGGCGAGTTTGAGGTCA |
| Suggested primer 2: | GGTCAACACGTGTGCTTTCC |