Insertion junction: CLIP2.025260_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025260
Chromosome:chromosome 9
Location:3907236
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g398067 FKB62,ROF1 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516:SF324 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP62 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCTACCCGCACAGGACGTGAAGCGCGCTCTGGAGCTGGAGCCGGACAG
Internal bar code:CGAGTGGCTGTTTATGTGCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:950
LEAP-Seq percent confirming:96.4286
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCAGGAAAAGGGCTGATC
Suggested primer 2:ACATGTGTTCACGTGCTCCT