| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.025278 |
| Chromosome: | chromosome 6 |
| Location: | 7651735 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302100 | (1 of 1) PTHR12907//PTHR12907:SF10 - EGL NINE HOMOLOG-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACGATGCTTCAACACCTACCTTATCCCTGTTCCCTTCCGCGCCGCTC |
| Internal bar code: | TCTTCTAAGATCTATATCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1276 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATCCCTGCTCGTATCCC |
| Suggested primer 2: | ACAATGATCCACCTGCCCTG |