| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025316 |
| Chromosome: | chromosome 16 |
| Location: | 2118936 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657850 | SEC63 | (1 of 1) K09540 - translocation protein SEC63 (SEC63, DNAJC23); ER-targeted preprotein translocase subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACACGGCCGTCCAGACAGCCTTGCACTGCCTGTTTTACCGCTACTTA |
| Internal bar code: | ACTCGGTGCATAAGTAGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1753 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGAACATAGGGCTGCAC |
| Suggested primer 2: | AGGACTGAAGAGTGCGTGTG |