| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025332 |
| Chromosome: | chromosome 9 |
| Location: | 42106 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386137 | (1 of 1) PF06824 - Protein of unknown function (DUF1237) (DUF1237) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGGCGCAAGGGCGGCGCGGGTAAGCGCCTGTGTCAGCTCTGCTACGC |
| Internal bar code: | GACCCGGGCTGGCGTGCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1381 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTACAACATCCCCGTCAA |
| Suggested primer 2: | AGTTCGCTGTCCATGCAAGA |