Insertion junction: CLIP2.025333_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025333
Chromosome:chromosome 5
Location:240664
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g243150 Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGGATCAGGGGTCGCAAAGGCGCACTGCCGCCGCTACAGCCGCCGCCG
Internal bar code:AGCGACTCTACCGGAGGATTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1063
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCTCCCAAACCCCACTC
Suggested primer 2:CTGCCACTGCTACTGCTGAT