Insertion junction: CLIP2.025334_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025334
Chromosome:chromosome 6
Location:5220009
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g283900 (1 of 1) PTHR16007//PTHR16007:SF15 - EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED // TRANSMEMBRANE PROTEIN 45B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCGCACGCACTCACCTAAAGATGCCCTGCATCCAGTGGACGCCCCAC
Internal bar code:CGGTTTTATAGGTCGACAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2713
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGTTGTGCAAAAGCACTA
Suggested primer 2:CATACCCAACCAGGTCCACC