| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.025361 |
| Chromosome: | chromosome 16 |
| Location: | 5864530 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679600 | (1 of 1) K11092 - U2 small nuclear ribonucleoprotein A' (SNRPA1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAACGCACTCTCCACCCACCGCAGAACGGCGGCGCGGAGCAGGGCGC |
| Internal bar code: | AAGCTTAAGTCTTGGGAAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 858 |
| LEAP-Seq percent confirming: | 21.0526 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCCTACGCTGGTTAAAG |
| Suggested primer 2: | CGTTCACCCTCTCGTCAACA |