Insertion junction: CLIP2.025368_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025368
Chromosome:chromosome 1
Location:5053746
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g035150 (1 of 19) PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACACACACACACACACACACACACACACA
Internal bar code:TGAGTAGTCGGAGTGCACTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2773
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCACGTATCTCTCCACTC
Suggested primer 2:GCGTGTTGCAAAGGTTTTGC