| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025446 |
| Chromosome: | chromosome 16 |
| Location: | 1851673 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655450 | (1 of 1) PF14510 - ABC-transporter extracellular N-terminal (ABC_trans_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAACATCTCGACCAGCACGTTCTTGCGGCTCTGTGTGAACGTGGCCA |
| Internal bar code: | TTTTTTAACGCTTCTCCTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4473 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTGGCCCCTGCTTACTCC |
| Suggested primer 2: | ATCAAGGTGAGCGCAATGGA |