Insertion junction: CLIP2.025477_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.025477
Chromosome:chromosome 7
Location:110588
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g312850 (1 of 1) PTHR31621:SF1 - DUF679 DOMAIN MEMBRANE PROTEIN 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTTCAAATAAAAGAAGTCGTACACGCGGTCCTTGTCCTCGTCCGTGGG
Internal bar code:TGGGAACTTTGTTGGCGTGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3341
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGTAGAATCGCTCGTGC
Suggested primer 2:AACGGTGCAAAACAATCCCG