Insertion junction: CLIP2.025493_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.025493
Chromosome:chromosome 10
Location:6535128
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g465450 (1 of 2) 2.8.2.20 - Protein-tyrosine sulfotransferase / Tyrosylprotein sulfotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCCCAAGCGACGATCGCACCAGCAATAGCACCCCACAGCCACCAGGT
Internal bar code:CATGGAGTTAGCCCGGGGCCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:209
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGTGCTGGTCAAGGTGA
Suggested primer 2:AGAGTGTGTGCGAGAGTGTG